small acid-soluble spore protein (major alpha-type SASP)
function
protection of spore DNA
product
small acid-soluble spore protein (major alpha-type SASP)
Genomic Context
categories
[category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.3|Small acid-soluble spore proteins][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]Gene
Coordinates
3,025,445 3,025,654
Phenotypes of a mutant
a [gene|80B5DAED9B4BD015D877DB4819AB25FC5F165C6D|sspA] mutant is sensitive to ionizing radiation [Pubmed|24123749]a [gene|80B5DAED9B4BD015D877DB4819AB25FC5F165C6D|sspA] [gene|28F47ADECD376494878AE58B395B3A2616B6B5DF|sspB] double mutant is sensitive to blue light-induced DNA damage [pubmed|30054368]The protein
Protein family
alpha/beta-type SASP family (according to Swiss-Prot)Paralogous protein(s)
[protein|9E1B3A6289F2C94F9088FA14CE8939E208DE9FEA|SspC], [protein|AEFA4CE1588C7EC7FCB01312A172093D12B2B206|SspD], [protein|28F47ADECD376494878AE58B395B3A2616B6B5DF|SspB] Modification
phosphorylated on Ser-6, Ser-9-, Ser-47, and Ser-55 [Pubmed|26381121]Structure
[PDB|2Z3X] ([protein|9E1B3A6289F2C94F9088FA14CE8939E208DE9FEA|SspC] in complex with DNA, 83% identity)Expression and Regulation
Operons
genes
[gene|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|nifZ]-[gene|A64A59C2B15015F13911EC53763D370F780400FD|thiI]-[gene|80B5DAED9B4BD015D877DB4819AB25FC5F165C6D|sspA]
description
[Pubmed|22383849]
view in new tabgenes
[gene|80B5DAED9B4BD015D877DB4819AB25FC5F165C6D|sspA]
description
[Pubmed|3009398]
sigma factors
[protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|15699190], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]regulatory mechanism
[protein|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|SpoVT]: activation, [Pubmed|8755877], in [regulon|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|SpoVT regulon]regulation
expressed late during sporulation in the forespore ([protein|search|SigG], [SW|SpoVT]) [Pubmed|15699190,8755877]additional information
the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]view in new tabBiological materials
Mutant
BP137 (''[gene|80B5DAED9B4BD015D877DB4819AB25FC5F165C6D|sspA]''::''cat'') available in [SW|Fabian Commichau]'s lab1S109 (no resistance), available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S109&Search=1S109 BGSC]1S111 (no resistance), [Pubmed|3087950], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S111&Search=1S111 BGSC]1S112 (no resistance), [Pubmed|3125155], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S112&Search=1S112 BGSC]1S113 (no resistance), [Pubmed|3125155], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S113&Search=1S113 BGSC]BKE29570 ([gene|80B5DAED9B4BD015D877DB4819AB25FC5F165C6D|sspA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGTCTCACCTCCTTGT, downstream forward: _UP4_TAATTTACAATTTCACATAABKK29570 ([gene|80B5DAED9B4BD015D877DB4819AB25FC5F165C6D|sspA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGTCTCACCTCCTTGT, downstream forward: _UP4_TAATTTACAATTTCACATAAReferences
16707666,2456528,3009398,12884008,11092849,7608092,15458366,19542328,15699190,8755877,23064347,3087950,3125155,24123749,26381121,30054368